Biosurfactant Production from Brewery Effluent Using Pseudomonas Spp. : Current School News

Comparative Analysis of Biosurfactant Production from Brewery Effluent Using Pseudomonas Species (Pseudomonas Aeruginosa and Pseudomonas Fluorescens)

Filed in Current Projects, Microbiology Project Topics by on September 30, 2022

Comparative Analysis of Biosurfactant Production from Brewery Effluent Using Pseudomonas Species (Pseudomonas Aeruginosa and Pseudomonas Fluorescens).


The increasing demand for surfactant calls for an alternative to the chemically synthesized surfactant, which poses a lot of threat to the environment due to bio-accumulation of its undegradable toxic component.

Accumulation of industrial and domestic waste also threatens the environment by contributing unfriendly gases and toxic compounds, therefore due to tighten environmental regulations and increasing need to protect the ecosystem, alternatives to chemically synthesized surfactant, which is biosurfactant, is a necessity.

Biosurfactants are biodegradable and stable at extreme temperatures and pH. Brewery effluent is an excellent substrate for the production of biosurfactant.

Pseudomonas aeruginosa and Pseudomonas fluorescens isolated from soil produced glycolipid biosurfactant from brewery effluent.

The brewery effluent used as substrate had chemical oxygen demand of 15,000mg/l, dissolved oxygen of 2.9mg/l and biological oxygen demand of 1.8mg/l.

Screening both isolates for their ability to produce biosurfactant, both were positive for drop collapse assay and hemolytic test.


Biosurfactants are surface active agents. They are amphiphilic biological compounds produced extracellularlly or as part of cell membrane by a variety of yeasts, bacteria and filamentous fungi (Rismani et al., 2006; Mata-Sandoval et al., 2002; Adebusoye et al., 2008).

Biosurfactant is a structurally diverse group of surface-active molecules synthesized by microorganisms. Their capability for reducing surface and interfacial tension with low toxicity and high specificity and biodegradability, led to an increasing interest in these microbial products as alternatives to chemical surfactants (Banat et al., 2000).

From the technical insights, it was estimated that biosurfactants could capture 10% of the surfactant market by the year 2010 with sales of $US200 million.

However, up to now, biosurfactants are still unable to compete with the chemically synthesized surfactants in the surfactant market.

This could be due to their high production costs in relation to inefficient bioprocessing methods available, poor strain productivity and the need to use expensive substrates (Cameotra and Makkar, 1998). 


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