Ads: Get Admission into 200 Level and Study any Course in any University of Your Choice. Low Fees | No JAMB UTME. Call 09038456231

Pharmacokinetic Interactions between Bioenhancers of Herbal Origin and Antiretroviral Drugs using Cytochrome P450 3A Inhibition Approach

ADS! Diabetes Permanent Solution! Only 15 Packs Left. HURRY!!!


Pharmacokinetic Interactions between Bioenhancers of Herbal Origin and Antiretroviral Drugs using Cytochrome P450 3A Inhibition Approach.

Abstract

The co-administration of oral therapeutic agents with natural compounds is currently on the increase with resultant increase in herb-drug interaction cases involving mainly cytochrome P450 enzymes and drug transporters.

HIV infection because of its  chronic  and  comorbid nature often necessitate the use of multiple drugs including herbs to relief symptoms.

Meanwhile, most natural products have been shown to modulate drug metabolising enzymes, resulting in alteration in co-administered drug concentrations.

The aims  of  this  study therefore, were to evaluate the in vitro and in vivo effects  of some  identified  herb  extracts used by HIV-infected patients on liver and intestinal cytochrome P450 3A enzymes  and  also to determine the extracts pretreatment effect on the bioavailability of some antiretroviral  agents.

The study adopted both survey and exprimental designs.A cross  sectional  study  was conducted in HIV clinic using a 25-item adapted questionnaire to identify the prevalence and pattern of herbal drug use among HIV infected patients on antiretroviral therapy.

Some of the identified herbs were collected and extracted using aqueous and non-aqueous solvents and concentrated using lyophilizer and rotary evaporator respectively.

In vitro inhibition of the cytochrome P450 3A enzymes by the extracts were assessed in liver  and  intestinal  microsomes using erythromycin-N-demethylation assay (EMND) method while their in vivo effects were determined by estimating simvastatin (CYP3A probe substrate) plasma concentrations in female rate model.

The effect of the extracts on two antiretroviral agents’ pharmacokinetics were determined by the administrations of the drug and the extracts to different rats divided into different groups of 5 rats per group and their plasma drug concentrations determined at different time intervals  using  HPLC.

Pharmacokinetic parameters were determined using non-compartmental anaysis as implemented in WinNonlin pharmacokineticsoftware application.

The effects of the extracts and the antiretroviral agents, administered concurrently, on hematological and biochemical parameters were determined.

HPLC fingerprinting assay was done to determine the extracts chemical constituents.  All results were presented as mean±SEM.

The data were subjected to one-way  analysis  of  variance (ANOVA) test and group differences determined using post hoc test (LSD) using SPSS. Results were considered statistically significant at P<0.05.

Introduction

Background Of Study

Until recently, all medicines were derived from natural materials (Sionneau & Pao, 1995) including those from earth, plant and animal origins.

Herbal medicines paved way for the conventional medicine with most drugs used in orthodox medicine originally derived from plant and animal materials.

The term herb/herbal products is commonly used for all preparations obtained from herbs, spices, roots,  leaves  and other non-botanical materials of natural origin (Obodozie, 2012).

Although, its name and natural source suggests something that is beneficial and has little potential for  harm, numerous toxic materials, such as foxglove, deadly nightshade and jimson weed (datura), have been known.

However, practitioners sometimes process the herbs to change them from their original form (Darling, 2002), and even isolate some active constituents, so that the end products are often not as nature presented them may be to reduce or abolish the toxicity.

For example, aconite  was processed  extensively in China to reduce its toxicity so that it could readily be used, and borneol, the active constituent found in a few tropical plants, was isolated centuries ago in relatively pure form, for both internal and external use (Leake, 1975).

Herbal medicine therefore, refers to all preparations gotten from herbs, spices, roots, stems, leaves and other non-botanical materials of natural origin which can be used for its therapeutic purposes.

These preparations may be used either as topical agents or for internal use. Herbal medicine is an integral part of Traditional medicine  which  has been defined by the World Health Organization (WHO) as the total of the knowledge, skills, and practices based on the theories.

Beliefs, and experiences indigenous to different cultures, whether explicable or not, used in the maintenance of health as well as in the prevention, diagnosis, improvement or treatment of physical and  mental illness (WHO, n.d.).

The World Health Organization’s estimate that 65-80 % of the population in developing countries relies on herbal medicines as primary source of treatment (Rahman & Singhal, 2002), points to the importance of this form of  medicine.

References

Achinewhu S.C., Ogbonna C.C., Hart A.D. (1995). Chemical composition of indigenous wild herbs, spices, fruits, nuts and leafy vegetable used as food. J.  Plant Food Hum. Nutr. 48, 341-388.

Adedayo, B. C. & Akindahunsi, A. A. (2010).Changes in the total phenol content and antioxidant properties of pepperfruit (Dennettia tripetala) with ripening.  Afr  J Food Sci., 4(6), 403-409

Adefegha, S. A. & Oboh, G. (2012). Inhibition of key enzymes linked to type  2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water extractable phytochemicals from some tropical spices. Pharm Biol., 50(7),857-65

Adonu, C. C., Ugwueze, M. E. and Ozioko, C. A. (2013). Phytochemical analyses of ethanol and water extracts of Millettia aboensis, Cuscuta reflexa, Daniella oliveri and synclisa scabrida. Inter J Pharm & Bio Sci, 3(2), 64-69

Agbakwuru, E.O.P., Osisiogu, I.U.W. and Riicker, G. (1979). Nig. J. Pharm. 10, (5 and 6), 203-205.

Agoha, R. C. (1974). Medicinal Plant of Nigeria. Offsetdikker Facuitect Waskunden Natnurwenten Schoppen. pp 22–158.

Enter your email address:

Delivered by TMLT NIGERIA

Join Over 3,500 000+ Readers Online Now!


=> FOLLOW US ON INSTAGRAM | FACEBOOK & TWITTER FOR LATEST UPDATES

ADS: KNOCK-OFF DIABETES IN JUST 60 DAYS! - ORDER YOURS HERE

COPYRIGHT WARNING! Contents on this website may not be republished, reproduced, redistributed either in whole or in part without due permission or acknowledgement. All contents are protected by DMCA.
The content on this site is posted with good intentions. If you own this content & believe your copyright was violated or infringed, make sure you contact us at [[email protected]] to file a complaint and actions will be taken immediately.

Tags: , , , , , , ,

Comments are closed.