Plasmid Profile of Antibiotic Resistant Salomonella Spp Isolated From Poultry Product
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ABSTRACT
The antibacterial resistance patterns of salmonella species isolated from poultry droppings in imo state university of owerri (imsu) the samples comprises of egg shell, soil in poultry farm feather, faces, clocal swab were analyzed microbiologically.
Isolation of salmonella was carried out on salmonella Shigella agar medium and identified according to their microscope and biochemical reaction bacterial isolates were obtained, identified and confirmed as salmonella species as identified from several biochemical examinations.
The isolates were subtested to antibiotics susceptibility test using disc diffusion test on Mueller Hinton Agar. High resistance was recorded against septrin(100%) chloramphednicol (100%), streptomycin (100%), taruid (100%), auginetin (100%).
Sparfloxacin(66%), ciprofloxacin (66%), Amoxacillon(91%), Gentermicin(91%) and pefloxacon(91%) plasmid profile was also carried out to determine and characterized antibiotics traits in these results reveal the presence of drug resistant salmonella in commercial poultry feeds in owerrl metropolis.
This poultry farm feather, faeces, can serve as a channel for transfer of resistant strains of bacteria to poultry birds as well as human and the environment feather, faeces, can serve as a channel for transfer of resistant strains of bacteria to poultry birds as well as human and the environment.
INTRODUCTION
Food-borne disease and food poisoning have increasily become a heath concern worldwide with species of salmonella spp. have for long been reported as leading cause of food borne infections (Whitwort et al., 2008).
In developed and developing countries salmonella serotypes have been extensively incriminated as the most important zoonotic pathogens in several countries worldwide (Akinyemi et al., 2007).
They are responsible for the significant morbidity and mortality in both humans and animals (Akinyemi et al., 2007), each year an estimated 1.3billion cases resulting to about 3million death occur worldwide due to salmonellosis alone (winkor et al., 2000).
In spite of the important of poultry as the major element in the human food chain, it has been frequently labeled as one of the most important sources of food poisoning due to salmonella serovars causing the majority of food borne outbreaks worldwide (European food safety agency., 2004).
Because of the importance of salmonella spp. As the cause of a food-borne disease, typing methods such as plasmid profiling have been used to trace the outbreak to the contaminated source for public health intervention (Akinyemi et al., 2007).
Plasmid analysis has recently been used to investigate an outbreak of multi resistant (R-type Acssuspi) salmonella isolates from poultry products or used in solving outbreaks caused by other salmonella and hygiene as well as immunization and proper nutrition has provided major benefit in human life expectancy ( world health organization; 2002).
However the increased utilization of antibiotics in both public and veterinary setting has led to the emergence of antibiotic resistance and as a conservence poses a serious threat to public health safety (world health organization: 2002).
REFERENCES
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